α-Anomeric Deoxynucleotides, Anoxic Products of Ionizing Radiation, Are Substrates for the Endonuclease IV-Type AP Endonucleases†
Identifieur interne : 000212 ( France/Analysis ); précédent : 000211; suivant : 000213α-Anomeric Deoxynucleotides, Anoxic Products of Ionizing Radiation, Are Substrates for the Endonuclease IV-Type AP Endonucleases†
Auteurs : Alexander A. Ishchenko [Russie] ; Hiroshi Ide [Russie] ; Dindial Ramotar [Russie] ; Georgy Nevinsky [Russie] ; Murat Saparbaev [Russie, France]Source :
- Biochemistry [ 0006-2960 ] ; 2004.
Abstract
α-Anomeric 2‘-deoxynucleosides (αdN) are one of the products formed by ionizing radiation (IR) in DNA under anoxic conditions. α-2‘-Deoxyadenosine (αdA) and α-thymidine (αT) are not recognized by DNA glycosylases, and are likely removed by the alternative nucleotide incision repair (NIR) pathway. Indeed, it has been shown that αdA is a substrate for the Escherichia coli Nfo and human Ape1 proteins. However, the repair pathway for removal of αdA and other αdN in yeast is unknown. Here we report that αdA when present in DNA is recognized by the Saccharomyces cerevisiae Apn1 protein, a homologue of Nfo. Furthermore, αT is a substrate for Nfo and Apn1. Kinetic constants indicate that αdA and αT are equally good substrates, as a tetrahydrofuranyl (THF) residue, for Nfo and Apn1. Using E. coli and S. cerevisiae cell-free extracts, we have further substantiated the role of the nfo and apn1 gene products in the repair of αdN. Surprisingly, we found that bacteria and yeast NIR-deficient mutants are not sensitive to IR, suggesting that DNA strand breaks with terminal 3‘-blocking groups rather than αdN might contribute to cell survival. We propose that the novel substrate specificities of Nfo and Apn1 play an important role in counteracting oxidative DNA base damage.
Url:
DOI: 10.1021/bi049214+
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 002176
- to stream Istex, to step Curation: 002176
- to stream Istex, to step Checkpoint: 000B40
- to stream Main, to step Merge: 003114
- to stream Main, to step Curation: 003082
- to stream Main, to step Exploration: 003082
- to stream France, to step Extraction: 000212
Links to Exploration step
ISTEX:30D697E1F68191E93F1439DEA40501EF7CAA1086Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>α-Anomeric Deoxynucleotides, Anoxic Products of Ionizing Radiation, Are Substrates for the Endonuclease IV-Type AP Endonucleases†</title><author><name sortKey="Ishchenko, Alexander A" sort="Ishchenko, Alexander A" uniqKey="Ishchenko A" first="Alexander A." last="Ishchenko">Alexander A. Ishchenko</name></author><author><name sortKey="Ide, Hiroshi" sort="Ide, Hiroshi" uniqKey="Ide H" first="Hiroshi" last="Ide">Hiroshi Ide</name></author><author><name sortKey="Ramotar, Dindial" sort="Ramotar, Dindial" uniqKey="Ramotar D" first="Dindial" last="Ramotar">Dindial Ramotar</name></author><author><name sortKey="Nevinsky, Georgy" sort="Nevinsky, Georgy" uniqKey="Nevinsky G" first="Georgy" last="Nevinsky">Georgy Nevinsky</name></author><author><name sortKey="Saparbaev, Murat" sort="Saparbaev, Murat" uniqKey="Saparbaev M" first="Murat" last="Saparbaev">Murat Saparbaev</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:30D697E1F68191E93F1439DEA40501EF7CAA1086</idno><date when="2004" year="2004">2004</date><idno type="doi">10.1021/bi049214+</idno><idno type="url">https://api.istex.fr/ark:/67375/TPS-DJ8HZ4PF-R/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">002176</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">002176</idno><idno type="wicri:Area/Istex/Curation">002176</idno><idno type="wicri:Area/Istex/Checkpoint">000B40</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000B40</idno><idno type="wicri:doubleKey">0006-2960:2004:Ishchenko A:anomeric:deoxynucleotides:anoxic</idno><idno type="wicri:Area/Main/Merge">003114</idno><idno type="wicri:Area/Main/Curation">003082</idno><idno type="wicri:Area/Main/Exploration">003082</idno><idno type="wicri:Area/France/Extraction">000212</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">α-Anomeric Deoxynucleotides, Anoxic Products of Ionizing Radiation, Are
Substrates for the Endonuclease IV-Type AP Endonucleases<ref type="bib" target="#bi0492141AF2"><hi rend="superscript">†</hi></ref></title><author><name sortKey="Ishchenko, Alexander A" sort="Ishchenko, Alexander A" uniqKey="Ishchenko A" first="Alexander A." last="Ishchenko">Alexander A. Ishchenko</name><affiliation wicri:level="1"><country xml:lang="fr">Russie</country><wicri:regionArea>Groupe “Réparation de l'ADN”, UMR 8126 CNRS, Institut Gustave Roussy, 94805 Villejuif Cedex, France,Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University,Higashi-Hiroshima 739-8526, Japan, Guy-Bernier Research Centre, University of Montreal, 5415 de l'Assomption,Montreal, Quebec H1T 2M4, Canada, and Novosibirsk Institute of Chemical Biology and Fundamental Medicine, SiberianDivision of the Russian Academy of Sciences, 630090 Novosibirsk</wicri:regionArea><wicri:noRegion>630090 Novosibirsk</wicri:noRegion></affiliation><affiliation></affiliation></author><author><name sortKey="Ide, Hiroshi" sort="Ide, Hiroshi" uniqKey="Ide H" first="Hiroshi" last="Ide">Hiroshi Ide</name><affiliation wicri:level="1"><country xml:lang="fr">Russie</country><wicri:regionArea>Groupe “Réparation de l'ADN”, UMR 8126 CNRS, Institut Gustave Roussy, 94805 Villejuif Cedex, France,Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University,Higashi-Hiroshima 739-8526, Japan, Guy-Bernier Research Centre, University of Montreal, 5415 de l'Assomption,Montreal, Quebec H1T 2M4, Canada, and Novosibirsk Institute of Chemical Biology and Fundamental Medicine, SiberianDivision of the Russian Academy of Sciences, 630090 Novosibirsk</wicri:regionArea><wicri:noRegion>630090 Novosibirsk</wicri:noRegion></affiliation><affiliation></affiliation></author><author><name sortKey="Ramotar, Dindial" sort="Ramotar, Dindial" uniqKey="Ramotar D" first="Dindial" last="Ramotar">Dindial Ramotar</name><affiliation wicri:level="1"><country xml:lang="fr">Russie</country><wicri:regionArea>Groupe “Réparation de l'ADN”, UMR 8126 CNRS, Institut Gustave Roussy, 94805 Villejuif Cedex, France,Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University,Higashi-Hiroshima 739-8526, Japan, Guy-Bernier Research Centre, University of Montreal, 5415 de l'Assomption,Montreal, Quebec H1T 2M4, Canada, and Novosibirsk Institute of Chemical Biology and Fundamental Medicine, SiberianDivision of the Russian Academy of Sciences, 630090 Novosibirsk</wicri:regionArea><wicri:noRegion>630090 Novosibirsk</wicri:noRegion></affiliation><affiliation></affiliation></author><author><name sortKey="Nevinsky, Georgy" sort="Nevinsky, Georgy" uniqKey="Nevinsky G" first="Georgy" last="Nevinsky">Georgy Nevinsky</name><affiliation wicri:level="1"><country xml:lang="fr">Russie</country><wicri:regionArea>Groupe “Réparation de l'ADN”, UMR 8126 CNRS, Institut Gustave Roussy, 94805 Villejuif Cedex, France,Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University,Higashi-Hiroshima 739-8526, Japan, Guy-Bernier Research Centre, University of Montreal, 5415 de l'Assomption,Montreal, Quebec H1T 2M4, Canada, and Novosibirsk Institute of Chemical Biology and Fundamental Medicine, SiberianDivision of the Russian Academy of Sciences, 630090 Novosibirsk</wicri:regionArea><wicri:noRegion>630090 Novosibirsk</wicri:noRegion></affiliation><affiliation></affiliation></author><author><name sortKey="Saparbaev, Murat" sort="Saparbaev, Murat" uniqKey="Saparbaev M" first="Murat" last="Saparbaev">Murat Saparbaev</name><affiliation wicri:level="1"><country xml:lang="fr">Russie</country><wicri:regionArea>Groupe “Réparation de l'ADN”, UMR 8126 CNRS, Institut Gustave Roussy, 94805 Villejuif Cedex, France,Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University,Higashi-Hiroshima 739-8526, Japan, Guy-Bernier Research Centre, University of Montreal, 5415 de l'Assomption,Montreal, Quebec H1T 2M4, Canada, and Novosibirsk Institute of Chemical Biology and Fundamental Medicine, SiberianDivision of the Russian Academy of Sciences, 630090 Novosibirsk</wicri:regionArea><wicri:noRegion>630090 Novosibirsk</wicri:noRegion></affiliation><affiliation></affiliation><affiliation wicri:level="1"><country wicri:rule="url">France</country></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Biochemistry</title><title level="j" type="abbrev">Biochemistry</title><idno type="ISSN">0006-2960</idno><idno type="eISSN">1520-4995</idno><imprint><publisher>American Chemical Society</publisher><date type="e-published">2004</date><date type="published">2004</date><biblScope unit="vol">43</biblScope><biblScope unit="issue">48</biblScope><biblScope unit="page" from="15210">15210</biblScope><biblScope unit="page" to="15216">15216</biblScope></imprint><idno type="ISSN">0006-2960</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0006-2960</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">α-Anomeric 2‘-deoxynucleosides (αdN) are one of the products formed by ionizing radiation (IR) in DNA under anoxic conditions. α-2‘-Deoxyadenosine (αdA) and α-thymidine (αT) are not recognized by DNA glycosylases, and are likely removed by the alternative nucleotide incision repair (NIR) pathway. Indeed, it has been shown that αdA is a substrate for the Escherichia coli Nfo and human Ape1 proteins. However, the repair pathway for removal of αdA and other αdN in yeast is unknown. Here we report that αdA when present in DNA is recognized by the Saccharomyces cerevisiae Apn1 protein, a homologue of Nfo. Furthermore, αT is a substrate for Nfo and Apn1. Kinetic constants indicate that αdA and αT are equally good substrates, as a tetrahydrofuranyl (THF) residue, for Nfo and Apn1. Using E. coli and S. cerevisiae cell-free extracts, we have further substantiated the role of the nfo and apn1 gene products in the repair of αdN. Surprisingly, we found that bacteria and yeast NIR-deficient mutants are not sensitive to IR, suggesting that DNA strand breaks with terminal 3‘-blocking groups rather than αdN might contribute to cell survival. We propose that the novel substrate specificities of Nfo and Apn1 play an important role in counteracting oxidative DNA base damage.</div></front></TEI><affiliations><list><country><li>France</li><li>Russie</li></country></list><tree><country name="Russie"><noRegion><name sortKey="Ishchenko, Alexander A" sort="Ishchenko, Alexander A" uniqKey="Ishchenko A" first="Alexander A." last="Ishchenko">Alexander A. Ishchenko</name></noRegion><name sortKey="Ide, Hiroshi" sort="Ide, Hiroshi" uniqKey="Ide H" first="Hiroshi" last="Ide">Hiroshi Ide</name><name sortKey="Nevinsky, Georgy" sort="Nevinsky, Georgy" uniqKey="Nevinsky G" first="Georgy" last="Nevinsky">Georgy Nevinsky</name><name sortKey="Ramotar, Dindial" sort="Ramotar, Dindial" uniqKey="Ramotar D" first="Dindial" last="Ramotar">Dindial Ramotar</name><name sortKey="Saparbaev, Murat" sort="Saparbaev, Murat" uniqKey="Saparbaev M" first="Murat" last="Saparbaev">Murat Saparbaev</name></country><country name="France"><noRegion><name sortKey="Saparbaev, Murat" sort="Saparbaev, Murat" uniqKey="Saparbaev M" first="Murat" last="Saparbaev">Murat Saparbaev</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/France/Analysis
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000212 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/France/Analysis/biblio.hfd -nk 000212 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= France
|étape= Analysis
|type= RBID
|clé= ISTEX:30D697E1F68191E93F1439DEA40501EF7CAA1086
|texte= α-Anomeric Deoxynucleotides, Anoxic Products of Ionizing Radiation, Are Substrates for the Endonuclease IV-Type AP Endonucleases†
}}
| This area was generated with Dilib version V0.6.33. |  |